Go to the bottom of the post to read the entire patent. RR
Patent History
Publication number: 20160090571
Type: Application
Filed: Sep 30, 2015
Publication Date: Mar 31, 2016
Applicant: Orior, LLC (Roseland, NJ)
Inventor: Clifford E. Carnicom (Wallace, ID)
Application Number: 14/871,544
US Patent Application for CULTURE MEDIA AND METHODS FOR CULTURING MICROORGANISMS Patent Application (Application #20160090571 issued March 31, 2016) - Justia Patents Search
https://patents.justia.com/patent/20160090571
Carnicom Patent March 31, 2016
Deep within the patent is found these words. RR
As shown herein, these microorganisms contain within them a highly polyunsaturated fat and/or fatty acids, that are expected to occur within the membranes of the microorganism. The microorganism may therefore be subject to, or result in, lipid peroxidation in the presence of free radicals. This process, once started, is a chain reaction and is only terminated in the presence of appropriate antioxidants, such as Vitamin E, glutathione peroxidase, transferrin (binding free iron), enzymes (such as catalase), in addition to others. Vitamin C and NAC (N-acetyl cysteine acting as a glutathione precursor) may be effective antioxidants as well.
Claims
30. A method for inhibiting growth of a microorganism associated with Morgellons disease, said method comprising contacting the microorganism with an inhibitor selected from the group consisting of an antioxidant of n-acetyl cysteine, glutathione, vitamin C and sodium citrate so that growth of the microorganism is inhibited.
I been using all these substances for some time now. Its worth the time and effort to read the entire patent. RR
What is peroxidase, transferrin? RR
ChatGPT
Peroxidase and transferrin are two different types of proteins, each with distinct functions and characteristics.
Peroxidase
Peroxidase is an enzyme that catalyzes the oxidation of various substrates by hydrogen peroxide. Here are some key points about peroxidases:
Function: Peroxidases play crucial roles in protecting cells from oxidative damage by breaking down hydrogen peroxide, a reactive oxygen species that can cause cellular damage.
Types: There are several types of peroxidases, including:
Horseradish Peroxidase (HRP): Commonly used in biochemistry and molecular biology for its role in enzyme-linked immunosorbent assays (ELISAs) and western blotting due to its ability to produce a colorimetric change when exposed to certain substrates.
Myeloperoxidase: Found in neutrophils, it contributes to the antimicrobial activities of these immune cells.
Glutathione Peroxidase: Protects cells from oxidative damage by reducing lipid hydroperoxides to their corresponding alcohols and reducing free hydrogen peroxide to water.
Biological Importance: Peroxidases are involved in various physiological processes, including defense against pathogens, hormone biosynthesis, and the metabolism of toxins.
What foods contain Peroxidase? If you ask the right questions you get the right answers. RR
ChatGPT
Peroxidase enzymes are found in a variety of foods, primarily in fruits, vegetables, and grains. Here are some common foods known to contain peroxidase:
Fruits:
Apples
Bananas
Pears
Grapes
Vegetables:
Broccoli
Cauliflower
Carrots
Potatoes
Turnips
Spinach
Brussels sprouts
Beets
Sweet potatoes
Grains and Legumes:
Wheat
Soybeans
Other Sources:
Horseradish (a particularly rich source of peroxidase, often used in commercial applications)
Peroxidase activity can vary significantly between different varieties and ripeness levels of these foods. The enzyme plays a role in the plant's response to stress and in the metabolism of hydrogen peroxide, making it more prevalent in fresh and minimally processed foods.
Transferrin
Transferrin is a glycoprotein that binds and transports iron in the blood. Here are some key points about transferrin:
Function: Transferrin is primarily responsible for the transport of iron from sites of absorption and storage to sites of utilization, such as the bone marrow, where iron is used for hemoglobin synthesis in red blood cells.
Iron Binding: Each transferrin molecule can bind two iron (Fe3+) ions. The iron-bound form is known as holo-transferrin, while the iron-free form is known as apo-transferrin.
Receptors: Cells have transferrin receptors on their surfaces to capture transferrin and extract iron from it. The transferrin receptor-mediated endocytosis is the primary mechanism for cellular iron uptake.
Clinical Significance: Measuring transferrin levels, as well as the percentage of transferrin saturation with iron, is important in diagnosing and managing conditions such as iron deficiency anemia and hemochromatosis (iron overload).
Both peroxidase and transferrin are essential for maintaining cellular and systemic homeostasis, though they function in very different biological contexts. Peroxidases are key in managing oxidative stress, while transferrin is crucial for iron metabolism and transport.
What foods contain Transferrin? If you ask the right questions you get the right answers. RR
ChatGPT
Transferrin is a protein primarily produced in the liver and is responsible for binding and transporting iron in the bloodstream. It is not directly present in foods. Instead, transferrin levels in the body can be influenced by dietary intake of iron and other nutrients that support iron metabolism.
To support healthy transferrin levels and overall iron metabolism, consider consuming foods that are rich in iron and nutrients that promote iron absorption:
Iron-rich Foods:
Red Meat: Beef, lamb, and pork are excellent sources of heme iron, which is easily absorbed by the body.
Poultry: Chicken and turkey also provide heme iron.
Fish and Shellfish: Fish like salmon, tuna, and shellfish such as clams, oysters, and shrimp are good sources of iron.
Legumes: Beans, lentils, chickpeas, and peas are high in non-heme iron.
Leafy Greens: Spinach, kale, and other dark leafy greens contain non-heme iron.
Nuts and Seeds: Pumpkin seeds, sesame seeds, and cashews are good sources of iron.
Fortified Foods: Many cereals, breads, and pastas are fortified with iron.
Foods that Enhance Iron Absorption:
Vitamin C-rich Foods: Citrus fruits (oranges, lemons, grapefruits), strawberries, bell peppers, and broccoli can enhance the absorption of non-heme iron.
Animal Proteins: Consuming meat, poultry, and fish along with plant-based iron sources can improve the absorption of non-heme iron.
Other Nutrients:
B Vitamins: Especially B6 and B12, which are found in meat, dairy, and fortified cereals.
Copper: Found in foods like liver, shellfish, nuts, and seeds, copper is important for iron metabolism.
By including a balanced diet with these foods, you can support your body’s production and regulation of transferrin and maintain healthy iron levels.
What is catalase? RR
ChatGPT
Catalase is an enzyme found in nearly all living organisms exposed to oxygen, such as bacteria, plants, and animals. Its primary function is to catalyze the decomposition of hydrogen peroxide into water and oxygen. This reaction is crucial because hydrogen peroxide is a toxic byproduct of various metabolic processes, and its accumulation can damage cells.
The chemical reaction catalyzed by catalase is: 2H2O2→2H2O+O22 H_2O_2 \rightarrow 2 H_2O + O_22H2​O2​→2H2​O+O2​
Here are some key points about catalase:
Structure: Catalase is a tetrameric enzyme, meaning it is composed of four subunits. Each subunit contains a heme group, which is vital for its enzymatic activity. The heme group contains iron, which plays a central role in the catalytic process.
Function: By breaking down hydrogen peroxide, catalase helps protect cells from oxidative damage. Hydrogen peroxide can react with iron or other metals to form highly reactive hydroxyl radicals, which can cause significant cellular damage.
Location: In eukaryotic cells, catalase is primarily found in peroxisomes, organelles that contain various enzymes involved in metabolic processes, including the detoxification of hydrogen peroxide.
Optimal Conditions: Catalase operates most effectively at a pH of around 7 (neutral) and at moderate temperatures typical of most biological systems.
Applications: Catalase has various applications, including:
Biological research: Used in studies involving oxidative stress and cell protection.
Industrial: Used in food preservation, textile processing, and waste treatment to break down hydrogen peroxide.
Medical: Potential therapeutic applications in conditions involving oxidative stress.
Understanding catalase's role and function helps in appreciating how organisms manage oxidative stress and maintain cellular health.
What foods contain Catalase? If you ask the right questions you get the right answers. RR
Catalase is an enzyme that helps decompose hydrogen peroxide into water and oxygen. It's found in nearly all living organisms exposed to oxygen. Foods that contain catalase include:
Fruits:
Apples
Bananas
Cherries
Apricots
Avocados
Vegetables:
Potatoes
Spinach
Carrots
Onions
Cabbage
Broccoli
Legumes:
Lentils
Peas
Beans
Meats and Animal Products:
Liver (especially beef liver, which is particularly high in catalase)
Kidneys
Red meat
Eggs
Grains and Nuts:
Whole grains
Wheat germ
Nuts and seeds
Fermented Foods:
Yogurt
Kefir
Sauerkraut
In general, fresh, raw foods are more likely to contain higher levels of catalase, as cooking can denature the enzyme and reduce its activity.
Very interesting info. I knew Clifford had his culturing/identification techniques, but didn’t know about his patent or the listed inhibitors.
Great questions and answers as always, thank you for sharing!
Curious if you have any thoughts on the DHF in comparison to this: https://substack.com/@kim4truth/note/c-59214271?r=2nof16&utm_medium=ios&utm_source=notes-share-action